Frequently Asked Questions - InVitroCYP Microsomes

What is the difference between M-class and H-class microsomes?

H-class microsomes are formulated to have high activity for use in inhibition studies. M-class microsomes are formulated to have average activity for use in metabolism studies.

How are the M-class and H-class microsomes manufactured?

M-class and H-class microsomes are purposed-pooled using a proprietary algorithm to ensure consistent and reproducible product across the two classes.

How are the InVitroCYP microsomes different from other microsomes?

The pooled microsome products that are available in the market today offer "average" cytochrome P450 (CYP) activity, which may not provide sufficient enzyme activity levels in some assays. The InVitroCYP classes provide differentiation so that researchers get the results they need.

How are the CYP enzyme levels reported?

InVitroCYPs are the only microsomes on the market with substrate data reported at both Km and Vmax, providing you with maximum visibility for predictive performance and quality.

What are C-class microsomes?

Because some studies look for specific interactions, InVitroCYP C-class microsomes are designed by you to fit your research criteria, e.g. microsomes prepared for specific donor demographics such as age, gender, race, CMV status etc.

What's the difference between Km and Vmax levels?

Km is measure of how easily the enzyme can be saturated by the substrate. Vmax is the maximum rate of an enzyme catalyzed reaction or when the enzyme is saturated by the substrate.

Why does BioreclamationIVT provide both Km and Vmax reports?

BioreclamationIVT used to only report Km values. However, many of our customers prefer Vmax values over Km. Km and Vmax values will be reported on all lot specific data sheets.