InVitroCYP™ H-class microsomes

InVitroCYP-H-Class-MicrosomesLiver microsomes provide a readily available and well-characterized biological model for use in CYP enzyme inhibition studies. InVitroCYP H-class microsomes are prepared from tissues that are screened and selected for high activity across the panel of relevant cytochrome P450 enzymes, including those CYPs that are traditionally low in activity. This microsome classification provides researchers with enhanced sensitivity on typically low activity CYPs.

 

  CYP Substrate Metabolites Units
AMO CYP2C8: Amodiaquine Decentralization Amodiaquine N-Desethylamodiaquine pmole/min/mg
BUP CYP2B6: Bupropion hydroxylation Bupropion Hydroxybupropion pmole/min/mg
COUM CYP2A6: Coumarin 7-hydroxylation Coumarin 7-HCG, 7-HCS, 7-HC pmole/min/mg
CZX CYP2E1: Chlorzoxazone 6-hydroxylation Chlorzoxazone 6-Hydroxychlorzoxazone pmole/min/mg
DEX CYP2D6: Dextromethorphan O-demethylation Dextromethorphan Dextrorphan pmole/min/mg
ECOD Multiple CYPs: 7-Ethoxycoumarin O-deethylation 7-Ethoxycoumarin 7-HCG, 7-HCS, 7-HC pmole/min/mg
MEPH CYP2C19: S-Mephenytoin 4'-hydroxylation S-Mephenytoin 4'-Hydroxymephenytoin pmole/min/mg
MID CYP3A4: Midazolam 1-hydroxylation Midazolam 1-Hydroxymidazolam pmole/min/mg
PHEN CYP1A2: Phenacetin O-deethylation Phenacetin Acetominophen pmole/min/mg
TEST CYP3A4: Testosterone 6ß-hydroxylation Testosterone 6β-Hydroxytestosterone pmole/min/mg
UGT UGT: 7-Hydroxycoumarin glucuronidation 7-Hydroxycoumarin 7-Hydroxycoumarin glucuronide pmole/min/mg
TOLB CYP2C9: Tolbutamide methyl-hydroxylation Tolbutamide 4'-Methylhydroxytolbutamide pmole/min/mg