Microsomes & S9

human-subcellular-fractionsThere are various subcellular fractions and they include homogenate, s9, microsomes and cytosol.  These products are valuable tools in drug discovery for studying the metabolism of your compounds.

Human Subcellular Products
  • InVitroCYP™ 150-Donor Human Liver Microsomes
  • InVitroCYP™ H-Class Human Liver Microsomes
  • InVitroCYP™ M-Class Human Liver Microsomes
  • Human Microsomes – Other Tissues
  • Human Liver S9

All microsomes and s9 products are tested by Quality Control to provide researchers with the best product in the market. Each preparation must pass set criteria for release.  Microsomes and S9 are tested for protein.  Human microsomes are tested for total P450. Enzyme characterization is performed on all human microsome and S9 products.

  CYP Substrate Metabolites Units
AMO CYP2C8: Amodiaquine Decentralization Amodiaquine N-Desethylamodiaquine pmole/min/mg
BUP CYP2B6: Bupropion hydroxylation Bupropion Hydroxybupropion pmole/min/mg
COUM CYP2A6: Coumarin 7-hydroxylation Coumarin 7-HCG, 7-HCS, 7-HC pmole/min/mg
CZX CYP2E1: Chlorzoxazone 6-hydroxylation Chlorzoxazone 6-Hydroxychlorzoxazone pmole/min/mg
DEX CYP2D6: Dextromethorphan O-demethylation Dextromethorphan Dextrorphan pmole/min/mg
ECOD Multiple CYPs: 7-Ethoxycoumarin O-deethylation 7-Ethoxycoumarin 7-HCG, 7-HCS, 7-HC pmole/min/mg
MEPH CYP2C19: S-Mephenytoin 4'-hydroxylation S-Mephenytoin 4'-Hydroxymephenytoin pmole/min/mg
MID CYP3A4: Midazolam 1-hydroxylation Midazolam 1-Hydroxymidazolam pmole/min/mg
PHEN CYP1A2: Phenacetin O-deethylation Phenacetin Acetominophen pmole/min/mg
TEST CYP3A4: Testosterone 6ß-hydroxylation Testosterone 6β-Hydroxytestosterone pmole/min/mg
UGT UGT: 7-Hydroxycoumarin glucuronidation 7-Hydroxycoumarin 7-Hydroxycoumarin glucuronide pmole/min/mg
TOLB CYP2C9: Tolbutamide methyl-hydroxylation Tolbutamide 4'-Methylhydroxytolbutamide pmole/min/mg